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目的:建立方法,同时测定茵栀黄颗粒中绿原酸、栀子苷、金丝桃苷和黄芩苷的含量。方法:选用Kromasil C18(4.6 mm×250 mm,5μm)柱;以乙腈-0.1%甲酸水溶液(37∶63)为流动相;流速为1.0 m L/min;柱温为35℃;检测波长程序:0~7.5 min为350 nm(绿原酸)、7.5~14 min为238 nm(栀子苷)、14~20 min为360 nm(金丝桃苷)、20~25 min为280 nm(黄芩苷)。结果:绿原酸、栀子苷、金丝桃苷和黄芩苷均具有良好的线性关系,线性范围分别为:0.3424~5.136μg、0.276~4.14μg、0.184~2.76μg、2.48~37.2μg;4种成分的平均加样回收率分别为98.1%(RSD=0.71%)、98.1%(RSD=1.07%)、98.0%(RSD=1.14%)、97.6%(RSD=0.95%)。结论:本含量测定方法准确高效,简便快速,适用于茵栀黄颗粒的质量评价。
Objective: To establish a method for simultaneous determination of chlorogenic acid, geniposide, hyperoside and baicalin in Yinzhihuang granule. Methods: Kromasil C18 (4.6 mm × 250 mm, 5 μm) column was used. The mobile phase was acetonitrile-0.1% formic acid solution (37:63), the flow rate was 1.0 m L / min and the column temperature was 35 ℃. 350 nm (chlorogenic acid) at 0-7.5 min, 238 nm (geniposide) at 7.5-14 min, 360 nm (hyperoside at 14-20 min, 280 nm at 20-25 min (baicalin ). Results: Chlorogenic acid, geniposide, hyperoside and baicalin had a good linear relationship, the linear range was 0.3424 ~ 5.136μg, 0.276 ~ 4.14μg, 0.184 ~ 2.76μg, 2.48 ~ 37.2μg; 4 The average recoveries of the components were 98.1% (RSD = 0.71%), 98.1% (RSD = 1.07%), 98.0% (RSD = 1.14%) and 97.6% (RSD = 0.95%), respectively. Conclusion: The method for determination of content is accurate and efficient, simple and rapid, suitable for the quality evaluation of Yinzhihuang granules.