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BCaCD_(885)细胞系是从一男性颊粘膜鳞状细胞癌患者原发灶所获得组织块,经体外培养2年已传180代以上。作者对原患者组织切片,体外培养不同代细胞和移植瘤用光镜、电镜进行观察,结果是三种细胞都具有鳞状上皮癌组织学特征。BCaCD_(885)细胞系的细胞生长快,失去接触性抑制,在软琼脂中形成克隆;其染色体众数为亚三倍体,畸变率高;在裸鼠体内具有成瘤性。BCaCD_(885)为已建成的恶性生物学特征稳定的确立细胞系。
The BCaCD_(885) cell line was obtained from a primary lesion of a male buccal mucosal squamous cell carcinoma patient and was passaged for more than 180 generations after 2 years of in vitro culture. The authors observed sections of the original patient’s tissue, in vitro cultured different generations of cells and transplanted tumors with light and electron microscopy. The result was that all three cells had histological features of squamous epithelial carcinoma. The cells of the BCaCD_(885) cell line grow rapidly, lose contact inhibition, and form clones in soft agar; the chromosomes are sub-triploid in frequency and have a high rate of aberrations; they have tumorigenicity in nude mice. BCaCD_(885) is a stable established cell line that has been established for malignant biological characteristics.