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Objective:To find out the effective treatment for infantile autumn diarrhea(IAD).Methods:Retention enema with Qilian Liquid(QLL) was applied to 106 patients of IAD with positive antigen of rotavirus in stool,and the result in the treated group was compared with that in the control group(82 patients) in aspects of clinical effect, negative conversion rate of rotavirus in stool and change of serum immunoglobulin. Animal experimental study was also conducted.Results:The disappearance time of principal symptoms, negative conversion rate of rotavirus in stool and serum levels of IgA and IgG in the treated group were significantly better than those in the control group (P<0.01, P< 0.05). Experimental study showed that QLL not only has the anti-viral effects to stop diarrhea, but also has effects in enhancing immune function and protecting intestinal mucous membrane. Conclusion:Retention enema with QLL is an effective therapy for the treatment of IAD.