采用根癌农杆菌介导大豆胚尖的遗传转化方法将番茄的LePT1基因导入辽豆17基因组中,以期获得能够高效利用磷素的转基因大豆新品种。PCR结果表明,T0和T1代植株基因组均扩增出1.6 kb的目的条带,测序并进行在线Blast比对表明该条带与LePT1基因相同,与试验预期相一致。结果表明LePT1基因已成功转入T0代植株,并在T1代大豆基因组中稳定遗传。 Genetic Transformation of Soybean Embryo Mediated by Agrobacterium tumefaciens The Lept1 gene of tomato was introduced into the genome of Liaodou 17 so as to obtain a new transgenic soybean that can utilize phosphorus efficiently. The results of PCR showed that 1.6 kb of target bands were amplified from the genome of T0 and T1 plants. Sequencing and online Blast alignment showed that the band was identical with LePT1 gene, which was consistent with the expected test. The results showed that LePT1 gene has been successfully transferred into T0 generation plants and stable inheritance in T1 generation soybean genome.