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The reduction of 4-nitrophenol catalyzed by nitroreductase in the presence of NADH was investigated in this paper.4-Amino- phenol and 4-bydroxylamino-phenol were found in the reductive products.The relationship between reaction time and the reductive ratio were studied.The similar reducing ratios of 4-nitrophenol were obtained under aerobic and anaerobic conditions.The results indicated that an oxygen-insensitive reaction was proceeded in the reduction of 4-nitrophenol and nitroreductase was an oxygen- insensitive enzyme.The reductive products of 4-nitrophenol were determined by HPLC and MS.
The reduction of 4-nitrophenol catalyzed by nitroreductase in the presence of NADH was investigated in this paper. 4-Amino-phenol and 4-bydroxylamino-phenol were found in the reductive products. The relationship between reaction time and the reductive ratio were studied. The similar reduction ratios of 4-nitrophenol were obtained under aerobic and anaerobic conditions. The results indicate that an oxygen-insensitive reaction was proceeded in the reduction of 4-nitrophenol and nitroreductase was an oxygen-insensitive enzyme. The reductive products of 4-nitrophenol were determined by HPLC and MS.