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取已鉴定的dPDLSCs细胞株复苏,倒置相差显微镜下观察细胞形态学及数量改变,平板培养并用吉姆萨染色计数克隆形成能力、体外诱导并用Von kossa染色观察体外分化特性,dPDLSCs与陶瓷化骨复合胶原凝胶载体三维培养条件下移植入免疫缺陷(BALB/C)小鼠皮下,分别4周、6周、8周取材,HE染色进行组织学观察。结果:dPDLSCs可在体外培养扩增,克隆形成能力为0.91%,体外矿化液诱导条件下,可向成骨方向分化,形成Von kossa染色阳性的矿化结节。
The identified cell line of dPDLSCs was resuscitated. Morphological and quantitative changes were observed under phase contrast microscope under inverted microscope. The ability of colony formation was evaluated by counting the colony forming ability with Giemsa staining. Von kossa staining was used to observe the differentiation in vitro. DPDLSCs and ceramic composite bone collagen Under three-dimensional culture conditions, the gel was transplanted subcutaneously into BALB / C mice for 4 weeks, 6 weeks and 8 weeks respectively. HE staining was used to observe the histological changes. RESULTS: The dPDLSCs could be cultured and expanded in vitro with a colony forming ability of 0.91%. Under the condition of mineralized fluid induction, dPDLSCs could differentiate into osteogenic direction and form Von kossa positive mineralized nodules.