目的　通过逆转录聚合酶链反应 (RT PCR)扩增SARS冠状病毒M蛋白基因 ,并构建M蛋白基因的原核表达载体 ,为进一步研究其功能做准备。方法　提取SARS冠状病毒总RNA ,通过RT PCR对其M蛋白基因进行扩增 ,并构建M蛋白基因的原核表达载体。结果　获得了SARS冠状病毒M蛋白基因的编码序列并将其克隆入原核表达载体pET30a。结论　成功构建SARS冠状病毒M蛋白基因的重组表达质粒 ,为进一步研究打下基础 Objective To amplify the M protein gene of SARS-CoV by reverse transcriptase-polymerase chain reaction (RT-PCR) and construct the prokaryotic expression vector of M protein gene in preparation for further study of its function. Methods The total RNA of SARS coronavirus was extracted and its M protein gene was amplified by RT PCR. The prokaryotic expression vector of M protein gene was constructed. Results The coding sequence of SARS-CoV M protein gene was obtained and cloned into prokaryotic expression vector pET30a. Conclusion The recombinant plasmid of M protein gene of SARS coronavirus was successfully constructed and laid the foundation for further study