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To clone HPV16 E2 gene from a biopsied cervical cancer sample Materials & Methods HPV16 E2 gene was amplified from specimen derived from a HPV 16 positive patient, then cloned and sequenced. Results The full length of HPV 16 E2 gene was successfully cloned. In comparison with the prototype accepted by GenBank, six point mutations in HPV 16 E2 nucleotide acid sequence were identified. Of them, three were missense, and one was in the overlapping E4 gene and was synonymous to E4. Conclusion HPV16 E2 gene was successfully cloned, and some nucleotide acids in its sequence were different from the prototype.
To clone HPV16 E2 gene from a biopsied cervical cancer sample Materials & Methods HPV16 E2 gene was amplified from specimen derived from a HPV 16 positive patient, then cloned and sequenced. Results The full length of HPV 16 E2 gene was successfully cloned. In comparison with the prototype accepted by GenBank, six point mutations in HPV 16 E2 nucleotide acid sequence were identified. Of them, three were missense, and one was in the overlapping was E4 gene and was synonymous to E4. Conclusion HPV16 E2 gene was successfully cloned, and some nucleotide acids in its sequence were different from the prototype.