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目的间充质干细胞(mesenchymal stem cells,MSCs)是存在于骨髓中的一种具有多向分化潜能和很强增殖能力的细胞。经静脉输注的MSCs能特异性地聚集于新生组织,如伤口和肿瘤等,本实验将MSCs作为基因治疗靶向载体,研究其在肿瘤组织内的抗血管作用。方法从雄性小鼠股骨骨髓中分离和培养MSCs,用流式细胞仪检测CD34、CD29、CD44的表达。再用sFlt-1(VEGF-R1)重组腺病毒感染后,给荷瘤小鼠静脉注射。结果间充质干细胞聚集在肿瘤内表达sFlt-1,使得肿瘤血管生成受抑制、肿瘤细胞凋亡增加、肺转移灶减小、生存时间延长。结论本研究表明,MSCs作为基因的运载工具在抗肿瘤领域可能有一定的应用价值。
Objective Mesenchymal stem cells (MSCs) are a type of cells with multidirectional differentiation potential and strong proliferative capacity in bone marrow. The MSCs that are transfused intravenously can specifically accumulate in new tissues, such as wounds and tumors. In this study, MSCs were used as gene therapy targeting vectors to study their anti-vascular effects in tumor tissues. Methods MSCs were isolated and cultured from bone marrow of femur of male mice. The expression of CD34, CD29 and CD44 was detected by flow cytometry. After re-sFlt-1 (VEGF-R1) recombinant adenovirus infection, to the tumor-bearing mice intravenously. Results Mesenchymal stem cells accumulated in the tumor expression of sFlt-1, making tumor angiogenesis inhibited, increased tumor cell apoptosis, reduced lung metastases, prolonged survival. Conclusion This study shows that MSCs as a carrier of gene may have some application value in anti-tumor field.