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地塞米松磷酸钠为多国药典所收载,其中其他甾体的检查方法有提取后紫外分光光度法及薄层层析法。本文采用高效液相色谱法分离出其他留体,用归一化法计算其他留体的总量并初步确证其中主要杂质为地塞米松。用内标法测定地塞米松磷酸钠的含量,测定结果较为满意。仪器及分析条件岛津LC-4A液相色谱仪;SPD-2AS紫外检测器,0.08AUFS,检测波长240nm;色谱柱YWG-G_(18)H_(37)10μmΦ4mm×250mm,柱效1250(以地塞米松磷酸钠峰计)。流动相0.01mol/L磷酸二
Dexamethasone sodium phosphate for the multi-national Pharmacopoeia included, among other methods for the examination of steroids after extraction UV spectrophotometry and thin-layer chromatography. In this paper, high performance liquid chromatographic separation of other retention, the normalization method to calculate the total amount of other retention and preliminary confirmed that the main impurity of which is dexamethasone. Determination of dexamethasone sodium phosphate content by internal standard method, the determination results are more satisfactory. Instrument and analysis conditions Shimadzu LC-4A liquid chromatograph; SPD-2AS UV detector, 0.08AUFS, detection wavelength of 240nm; column YWG-G 18 H 37 (37) 10μmΦ4mm × 250mm, efficiency 1250 The peak of dexamethasone sodium phosphate). Mobile phase 0.01mol / L phosphate two