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背景:Ola鼠是一种基因变异鼠,其周围神经轴突损伤后华勒氏变性速度比正常的6J鼠缓慢,增加损伤因素可能有助于了解Ola鼠的特性。目的:观察丙烯酰胺对捻挫损伤后C57BL/Ola(Ola)鼠和C57BL/6J(6J)鼠坐骨神经有髓纤维变性和再生的不同病理过程。设计:随机对照实验。单位:深圳市第二人民医院神经内科和吉林大学第一医院神经内科。材料:实验于1996-01/06在日本产业医科大学神经内科进行。选择成年Ola鼠和6J鼠各12只,Ola鼠和6J鼠各6只为实验组,其余各6只为对照组。方法:全部动物麻醉状态下暴露坐骨神经上段,用止血钳捻挫神经近端10s后缝合。实验组动物注射总剂量为350mg的丙烯酰胺,对照组动物同时腹腔注射等量生理盐水。坐骨神经捻挫损伤后14d,全部动物再次麻醉,取捻挫部位远端坐骨神经制备切片,测量并计算神经横截面积,有髓纤维密度和有髓纤维大小频率分布和每根神经的有髓纤维数目。主要观察指标:两组中01a鼠和6J鼠坐骨神经的有髓纤维密度、有髓纤维数目、有髓纤维最大直径、有髓纤维平均直径。结果:参加实验Ola鼠和6J鼠各12只全部进入结果分析。①Ola鼠没有发生华勒氏变性;而6J鼠可见有髓纤维变性和许多变性后新生小径有髓纤维。②实验组6J鼠坐骨神经总有髓纤维密度低于Ola鼠(P<0.05);总有髓纤维数目少于Ola鼠(P<0.01),以大径纤维数目减少明显(P<0.01);有髓纤维平均直径也明显小于01a鼠(P<0.01)。结论:Ola鼠捻挫伤后华勒氏变性速度极其缓慢,丙烯酰胺对这一特征没有影响;丙烯酰胺对6J鼠捻挫损伤后轴突的再生过程有抑制作用。
BACKGROUND: The Ola mouse is a mutant mouse that has a slower rate of Wahrer’s degeneration than the normal 6J rat after axonal injury. Increasing the damage factor may help to characterize the Ola rat. OBJECTIVE: To observe the different pathological processes of acrylamide on myelinated fibrosis and regeneration of the sciatic nerve in C57BL / Ola (Ola) and C57BL / 6J (6J) mice after wrestling. Design: Randomized controlled experiment. SETTING: Department of Neurology, Second People’s Hospital of Shenzhen and Department of Neurology, First Hospital of Jilin University. Materials: The experiment was performed at Department of Neurology, Nihon University of Medical Sciences from January 1996 to June 2006. Twelve adult Ola and 6J mice, six Ola mice and 6J mice were selected as the experimental group and the other six as the control group. Methods: All the animals were exposed to the upper part of the sciatic nerve under anesthesia, sutured the proximal end of the nerve with a hemostatic forceps for 10 seconds. Animals in the experimental group were injected with a total dose of 350 mg of acrylamide, while animals in the control group were injected intraperitoneally with the same amount of saline. At 14 days after sciatic nerve injury, all the animals were re-anesthetized. The distal sciatic nerve of the torsion area was sectioned and the nerve cross-sectional area, the density of myelinated fibers, the frequency distribution of myelinated fibers and the number of myelinated fibers in each nerve were measured and calculated. MAIN OUTCOME MEASURES: The myelinated fiber density, the number of myelinated fibers, the maximum diameter of myelinated fibers, and the mean diameter of myelinated fibers in the sciatic nerve of both 01a and 6J mice in both groups were compared. RESULTS: Twelve of the experimental Ola and 6J mice, respectively, entered the result analysis. ① Ola rats did not occur Waldorf’s degeneration; and 6J rats showed myelofibrosis and many degeneration of new trails of medullary fibers. ② The total myelinated fiber density of experimental group 6J was lower than that of Ola (P <0.05); the total number of myelinated fibers was less than that of Ola (P <0.01), and the number of large diameter fibers decreased significantly (P <0.01); The mean diameter of myelinated fibers was also significantly smaller than that of 01a rats (P <0.01). CONCLUSION: The rate of Wright’s degeneration after Oliver’s contusion is extremely slow. Acrylamide has no effect on this feature. Acrylamide has inhibitory effect on axon regeneration after 6w twisting.