目的对转基因人参CS83细胞系表达HBsAg的特异性、稳定性及表达效率进行分析。方法提取CS83细胞表达蛋白,分别以Lowry法和ELISA法检测总蛋白含量和HBsAg含量,SDS-PAGE和Western blot分析表达蛋白的HBsAg特异性。采用冷冻干燥、干燥和低温处理方法检测CS83细胞中HBsAg的稳定性。结果CS83细胞表达产物中总蛋白含量为2·024mg/g,HBsAg含量为504ng/g,占总蛋白含量的0·025%。表达的目的蛋白相对分子质量约为25000,具有HBsAg特异性。经不同方法处理后的保存结果表明,冻干保存稳定性最好。结论所建立的CS83细胞系能够稳定地表达特异性HBsAg。 Objective To analyze the specificity, stability and efficiency of expression of HBsAg in transgenic ginseng CS83 cell line. Methods The protein expression of CS83 cells was extracted. The total protein content and HBsAg content were detected by Lowry and ELISA respectively. The specificity of HBsAg protein was analyzed by SDS-PAGE and Western blot. The stability of HBsAg in CS83 cells was measured by freeze-drying, drying and cryogenic treatment. Results The total protein content of CS83 cells was 2.024 mg / g and the content of HBsAg was 504 ng / g, accounting for 0.025% of the total protein content. The expressed protein of interest has a relative molecular mass of about 25000 and has HBsAg specificity. The results of different treatments showed that the best storage stability was obtained. Conclusion The established CS83 cell line can stably express specific HBsAg.