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目的研究环孢素A(Cs A)对人胃癌BGC823细胞的生长增殖以及凋亡的影响。方法 MTT法检测不同浓度Cs A处理24、48、72 h后对BGC823细胞增殖抑制率;流式细胞术(FCM)检测细胞周期、活性氧(ROS)含量及细胞线粒体膜电位(△Ψm)改变;Annexin-V-FITC/PI双染法检测细胞凋亡率。结果 Cs A在5~20μmol/L浓度范围内和24~72 h时间范围对BGC823细胞增殖有显著抑制作用,与药物剂量、作用时间呈现量效和时效的依赖性;FCM结果显示Cs A浓度依耐性使细胞周期阻滞于G0/G1期,与对照组相比差异有统计学意义(F=33.45,P<0.05,P<0.01);细胞凋亡率随Cs A作用浓度增加而增加;5~20μmol/L Cs A浓度范围细胞内ROS含量显著增加(F=46.17,P<0.01),细胞线粒体膜电位明显降低。结论 Cs A对BGC823细胞有抑制增殖和诱导凋亡作用,其机制可能与阻滞细胞生长周期、细胞内ROS含量增加以及线粒体膜电位下降相关。
Objective To study the effect of CsA on the growth, proliferation and apoptosis of human gastric cancer BGC823 cells. Methods MTT method was used to detect the inhibitory rate of proliferation of BGC823 cells treated with different concentrations of CsA for 24,48,72 h. Cell cycle, reactive oxygen species (ROS) content and mitochondrial membrane potential (△ ψm) were detected by flow cytometry (FCM) Annexin-V-FITC / PI double staining was used to detect the apoptosis rate. Results The CsA concentration in the range of 5 ~ 20μmol / L and the time range of 24 ~ 72h significantly inhibited the proliferation of BGC823 cells, and dose-dependent and time-dependent dose-dependent and time-dependent manner. The results of FCM showed that the concentration of CsA was Tolerance blocked the cell cycle in G0 / G1 phase, which was significantly different from the control group (F = 33.45, P <0.05, P <0.01). The apoptotic rate increased with the increase of CsA concentration.5 The level of intracellular ROS increased significantly (~ 46.17, P <0.01) in the concentration range of ~ 20μmol / L CsA, and the mitochondrial membrane potential was significantly decreased. Conclusions CsA can inhibit proliferation and induce apoptosis of BGC823 cells. The mechanism may be related to the arrest of cell growth cycle, the increase of intracellular ROS, and the decrease of mitochondrial membrane potential.