6—4 反相色谱 反相色谱通常是指以具有非极性表面的担体为固定相,以比固定相极性更强的溶剂系统为流动相的色谱分离。一个典型的例子就是在十八烷基硅烷键合相上用甲醇/水混合溶剂冲洗。“反相色谱”是相对于“正相色谱”而言的,在后一种情况下,使用极性固定相(如硅胶)和非或弱极性的流动相 6-4 Reversed-phase chromatography Reversed-phase chromatography usually refers to the carrier with a non-polar surface as the stationary phase, with a more polar solvent system than stationary phase mobile phase chromatographic separation. A typical example is rinsing with octadecylsilane-bonded phases with a methanol / water mixed solvent. “Reversed phase chromatography” is relative to “normal phase chromatography,” in the latter case using a polar stationary phase (such as silica gel) and a mobile phase of either non-polar or weak polarity