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Objective: To investigate the effects of endothlin-1 (ET-1) on vascular smooth muscle cells(VSMCs) proliferation and the expression of Peroxisome proliferator-activated receptorγ (PPAR-γ) in VSMCs. Methods: VSMCs of 16-week-old wistar rats thoracic aorta were cultured. VSMCs were treated by ET-1 for 48 h and observed of the proliferation by MTT. The expression of PPAR-γ mRNA and protein in cultured VSMCs treated by different concentration of ET-1 for 48 h was detected by RT-PCR and Western blot. Results: Compared with control group, VSMCs treated by ET-1 proliferated with the increase concentration of ET-1. There was significant differences among different groups (P<0.01). Meanwhile,the expression of PPAR-γ both in mRNA and in protein levels deceased. The expression of PPAR-γ in VSMCs was gradually decreased along with the increase concentration of ET-1. There was significant differences among different groups (P<0.01). Compared with control group, the expression of both PPAR-γ mRNA and PPAR-γ in ET-1 treated groups were lower(P<0.01). Conclusion: ET-1 could induce VSMCs proliferation and the expression of PPAR-γ in VSMCs, which demonstrates that high dose ET-1 obviously weakens the function of PPAR-γ to increase VSMCs proliferation.
Objective: To investigate the effects of endothlin-1 (ET-1) on vascular smooth muscle cells (VSMCs) proliferation and the expression of Peroxisome proliferator-activated receptorγ (PPAR-γ) in VSMCs. Methods: VSMCs of 16-week- VSMCs were treated by ET-1 for 48 h and observed the proliferation of MTT. The expression of PPAR-γ mRNA and protein in cultured VSMCs treated by different concentration of ET-1 for 48 h was detected by RT-PCR and Western blot. Results: Compared with control group, VSMCs treated by ET-1 proliferated with the increase concentration of ET-1. There was significant differences among different groups (P <0.01). Meanwhile, the expression of PPAR The expression of PPAR-γ in VSMCs was gradually decreased along with the increase concentration of ET-1. There was significant differences among different groups (P <0.01). Compared with control group, the expression of both PPAR-γ m ET-1 could induce VSMCs proliferation and the expression of PPAR-γ in VSMCs, which demonstrates that high dose ET-1 obviously weakens the function of of PPAR-γ to increase VSMCs proliferation.