超广谱β-内酰胺酶(ESBLs)是革兰阴性杆菌对广谱头孢菌素以及单环β-内酰胺类抗生素产生耐药的最重要机制之一。缺少可靠的检测方法是造成产ESBLs细菌广泛流行的原因之一。人们探索了许多方法,如双纸片、E-test、等电聚焦电泳等方法。本文对目前应用的检测ESBLs的大部分方法作了综述。 Extended spectrum β-lactamase (ESBLs) is one of the most important mechanisms of resistance of gram-negative bacilli to broad-spectrum cephalosporins and monocyclic β-lactam antibiotics. The lack of reliable detection methods is one of the causes of the widespread epidemic of ESBLs-producing bacteria. People have explored many methods, such as double paper, E-test, isoelectric focusing electrophoresis and other methods. This review summarizes most of the currently used methods of detecting ESBLs.