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用Sephadex G200柱层析、聚丙烯酰胺凝胶电泳及荧光探针法,对热酚法制备的抗肿瘤iRNA制剂进行了组成与构象分析。抗肿瘤iRNA制剂是至少由4种RNA分子组成的,与未免疫处理的相应RNA制剂在组成含量及结构性质上均不同,且其构象不是螺旋度最高的。
Sephadex G200 column chromatography, polyacrylamide gel electrophoresis and fluorescent probe method were used to analyze the composition and conformation of anti-tumor iRNA preparations prepared by thermal phenol method. Anti-tumor iRNA preparations are composed of at least four RNA molecules, and the corresponding RNA preparations that are not immuno-treated have different compositional content and structural properties, and their conformations are not the highest in the degree of helicity.