论文部分内容阅读
目的:观察角叉菜炎症时,5-羟色胺2A(5-HT_(2A))受体mRNA在中缝大核(NRM)表达的水平及其与5-羟色胺(5-HT)免疫阳性神经元的共存。方法:实验采用原位杂交和荧光原位杂交结合荧光免疫组织化学双标技术。结果:正常大鼠NRM内有中等水平的5-HT_(2A)受体mRNA表达;角叉菜致炎后,5-HT_(2A)受体mRNA在双侧NRM内的表达明显增加,3-8h达峰值,24h后显著降低。在正常大鼠的NRM内,仅有少量5-HT_(2A)受体mRNA和5-HT免疫阳性双标神经元,双标神经元的数量在角叉菜致炎后无明显变化。结论:NRM内5-HT_(2A)受体主要分布在非5-HT神经元,且随外周炎症的发展,5-HT_(2A)受体的合成增加。
OBJECTIVE: To observe the expression of 5-HT 2A (5-HT 2A receptor) mRNA in the nucleus raphe magnus (NRM) and its relationship with 5-HT seropositive neurons coexist. Methods: In situ hybridization and fluorescence in situ hybridization combined with fluorescence immunohistochemistry double labeling technique. Results: The mRNA expression of 5-HT 2A receptors in NRM of normal rats was significantly increased after carrageenin-induced inflammation. The expression of 5- 8h peak, significantly reduced after 24h. There was only a small amount of 5-HT2A receptor mRNA and 5-HT immunopositive double labeled neurons in NRM of normal rats. The number of double labeled neurons did not change significantly after carrageenin-induced inflammation. CONCLUSIONS: The 5-HT 2A receptors are mainly distributed in non-5-HT neurons in NRM. The synthesis of 5-HT 2A receptor is increased with the development of peripheral inflammation.