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以甘薯(Ipomoea batatas L.)济黑1号为试材,对济黑1号茎尖脱毒和脱毒苗快繁技术进行研究,并用指示植物法对再生植株无性系进行病毒检测。结果表明,在薯块出芽30 d内,外植体的处理中采用2%的NaClO溶液处理最好,接种于含有1 mg/L 6-BA的MS培养基中,取材污染率最低,成苗率最高。快繁过程中普通MS培养基中加入0.02 mg/L IAA对快繁更加有利。
Taking Jihei 1 of sweet potato (Ipomoea batatas L.) as test material, the technology of detoxification and virus-free seedling extirpation of Jihei No.1 was studied, and the virus detection of the regenerated plant clones was carried out by the indicated plant method. The results showed that explants were treated best with 2% NaClO solution within 30 days after sprouting, and were inoculated into MS medium containing 1 mg / L 6-BA, with the lowest contamination rate and seedling emergence The highest rate. In the process of fast propagation, the addition of 0.02 mg / L IAA to ordinary MS medium is more beneficial to the propagation.