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目的观察益气化瘀清热方及拆方的含药血清对脂多糖刺激体外培养的大鼠肾小球系膜细胞(Ms C)增殖、生成Ⅰ型胶原蛋白(Col-Ⅰ)的影响。方法 MTT法检测Ms C增殖;ELISA法检测Col-Ⅰ的含量。结果各类含药血清均能抑制Ms C增殖、减少Col-Ⅰ的生成,化瘀组与清热组作用强于益气组,中药复方组作用强于雷公藤组。结论益气化瘀清热方及拆方后的各类中药均能抑制Ms C增殖、减少Col-Ⅰ的生成。
Objective To observe the effect of Yiqi Huayu Qingre recipe and its disassembled serum on proliferation and collagen type Ⅰ collagen Ⅰ (Col Ⅰ) induced by lipopolysaccharide in cultured rat glomerular mesangial cells (Ms C). Methods The proliferation of Ms C was detected by MTT assay. The content of Col-Ⅰ was detected by ELISA. Results All kinds of serum containing Ms C could inhibit the proliferation of Ms C and reduce the production of Col-Ⅰ. The effect of Huayu group and Qingre heat-treated group was stronger than that of Yiqi group. The effect of traditional Chinese medicine compound group was stronger than that of Tripterygium wilfordii group. Conclusion Yiqi Huayu Qingre prescription and all kinds of traditional Chinese medicine after disassembled can inhibit Ms C proliferation and reduce the formation of Col-Ⅰ.