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目的：检测乳腺癌及其癌变过程中细胞DNA的含量，探讨DNA含量在这一过程中的变化规律和临床意义。方法：对1988年63例在我院手术治疗的浸润性乳腺癌，以及1984～1992年间手术治疗的13例导管内癌（ductalcarcinomainsitu，DCIS）、6例导管内癌伴早期浸润（DCIS＋Ca）、6例导管上皮高度增生（proliferativebreastdisease，PBD）的原发灶进行DNA图象分析（imagecytometry，ICM）,结合临床资料，分析63例浸润性乳腺癌细胞DNA含量与预后关系的观察。结果：发现浸润性乳腺癌与DCIS及PBD相比，有较高的DNA指数（DNAindex，NA）DI)、异倍体检出率、核面积（nucleararea，NA）和5C细胞阳性率（P＜0.05）。在DCIS与PBD两组病人中，DI、NA无明显差别（P＜0.05），而异倍体肿瘤,5C细胞出现在DCIS中（P＜0.05），且PBD中无5C细胞、异倍体出现。在乳腺癌病人预后的单因素分析中，发现5C细胞阳性、异倍体肿瘤等的无病生存率低（P＜0.01）。结论:异倍体细胞群的出现是细胞癌变的早期标志，DNA含量随着乳腺癌恶性程度增加而发生显著改变。
OBJECTIVE: To detect the cellular DNA content in the process of breast cancer and its carcinogenesis, and to explore the changing rules and clinical significance of DNA content in this process. Methods: 63 cases of invasive breast cancer surgically treated in our hospital in 1988, and 13 cases of ductal carcinomas in situ (DCIS) treated surgically between 1984 and 1992, 6 cases of intraductal carcinoma with early infiltration (DCIS+Ca), 6 The primary tumors of proliferative breast-disease (PBD) primary lesions were subjected to DNA cytometric analysis (ICM) combined with clinical data to analyze the relationship between DNA content and prognosis of 63 invasive breast cancer cells. RESULTS: We found that compared with DCIS and PBD, invasive breast cancer had higher DNA index (NA) DI, heteroploid detection rate, nuclear area (NA), and positive rate of 5C cells (P<0.05). ). There was no significant difference in DI and NA between DCIS and PBD patients (P<0.05), while in heteroploid tumors, 5C cells appeared in DCIS (P<0.05), and no 5C cells and aneuploids appeared in PBD. . In the univariate analysis of prognosis of breast cancer patients, the disease-free survival rate of 5C-positive and aneuploid tumors was found to be low (P<0.01). Conclusion: The appearance of aneuploid cell clusters is an early sign of cell carcinogenesis, and the DNA content changes significantly with the increase in malignancy of breast cancer.