T_3、T_4定量测定是用来诊断甲状腺功能重要的指标。在大中型医院开展较为普遍,多年来各医院多采用放免法,此法具有高灵敏度,高特异性的优点,但也存在缺陷:如半衰期短,限制了试剂盒的使用寿命,由于标记物的不断变化,带来试剂批间、批内的较大变异,标准曲线无法保存备用,反应时间过长(数小时或过夜),另外放免法使用放射性核素对工作人员造成放射性损伤,需要一定的保护。而酶标法也具有较高的灵敏 T_3, T_4 quantitative determination is used to diagnose thyroid function important indicators. In large and medium-sized hospitals are more common, many hospitals over the years using radioimmunoassay, this method has the advantages of high sensitivity, high specificity, but there are shortcomings: such as short half-life, limiting the service life of the kit, due to the marker Continuous changes, resulting in reagent batch, batch variation within the larger, the standard curve can not be stored for long, the reaction time is too long (hours or overnight), and other radioactive nuclides radionuclide damage caused by staff, you need a certain protection. The enzyme method also has a high sensitivity