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目的:探讨趋化因子受体CXCR7在结肠直肠癌中的表达及其临床意义。方法:收集48例结肠直肠癌病人的癌组织和癌旁组织,采用实时定量PCR和免疫组化方法,分别检测CXCR7在mRNA及蛋白水平的表达。结果:CXCR7 mRNA表达在结肠直肠癌和相对应癌旁组织中的差异无统计学意义(P>0.05),与临床病理特征无明显相关;但结肠直肠癌中CXCR7蛋白的表达高于癌旁组织(P<0.001),且其表达与淋巴结转移、AJCC分期及肿瘤部位均有相关性(P值分别为0.024、0.024、0.010)。结论:CXCR7在结肠直肠癌中mRNA和蛋白水平上的表达有差异;CXCR7蛋白的表达与结肠直肠癌的进展及转移有关。
Objective: To investigate the expression of chemokine receptor CXCR7 in colorectal cancer and its clinical significance. Methods: Forty-eight patients with colorectal cancer were collected for cancer tissue and adjacent tissues. Real-time quantitative PCR and immunohistochemistry were used to detect the expression of CXCR7 at mRNA and protein levels. Results: There was no significant difference in the expression of CXCR7 mRNA between colorectal cancer and corresponding paracancerous tissues (P> 0.05), but not with clinicopathological features. However, the expression of CXCR7 protein in colorectal cancer was higher than that in paracancerous tissues (P <0.001), and the correlation between the expression and lymph node metastasis, AJCC staging and tumor location (P values were 0.024,0.024,0.010 respectively). Conclusion: CXCR7 expression in colorectal cancer mRNA and protein levels are different; CXCR7 protein expression and colorectal cancer progression and metastasis.