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目的:研究白羽鸡胚心肌细胞的膨胀激活氯电流及氯丙嗪对其的模拟作用.方法:膜片箝技术的全细胞记录模式.结果:低渗膨胀在白羽鸡胚心肌上诱发出一个可逆变化的氯电流,渗透压从300 mmol·L~(-1)减小至270 mmol·L~(-1)时,该电流从(452±200)pA增加到(849±373)pA.DIDS 100 μmol·L~(-1)使氯电流从(1196±505)pA减至(830±328)pA.I(Cl,swell)不能被CPZ 30μmol·L~(-1)模拟出来,该结果不同于大肠杆菌原生质体的结果.结论:白羽鸡胚心肌细胞的膨胀激活氯通道能被低渗激活,其激活的机制与大肠杆菌的机制敏感性离子通道激活的机制不同.
OBJECTIVE: To study the expansion-activated chloride currents of white chicken embryo cardiocytes and the effect of chlorpromazine on it.Methods: The whole-cell recording mode of patch clamp technique was used.Results: Hypotonic swelling induced a reversible The currents increased from (452 ± 200) pA to (849 ± 373) pA.DIDS when the change of chloride current and osmotic pressure decreased from 300 mmol·L -1 to 270 mmol·L -1. 100 μmol·L -1 decreased the chloride current from (1196 ± 505) pA to (830 ± 328) pA.I (Cl, swell) could not be simulated by CPZ 30 μmol·L -1, Which is different from that of E. coli protoplasts.Conclusion: The expansion-activated chloride channel of white chicken embryo myocytes can be activated by hypotonic mechanism, which is different from the mechanism of Escherichia coli’s mechanism-sensitive ion channel activation.