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作者以~3H-酪蛋白作为底物,测定变应性鼻炎和慢性鼻窦炎病人鼻分泌物中的蛋白酶和抗蛋白酶活性。发现慢性鼻窦炎脓性鼻分泌物中的蛋白酶活性明显高,而变应性鼻炎鼻分泌物中的这种酶活性则特别低。另一方面,变应性鼻炎鼻分泌物中胰蛋白酶抑制活性却明显高于鼻窦炎(P<0.01)。说明变应性鼻炎鼻分泌物中存在着很强的蛋白酶抑制因子。通过SDS-多丙基胺凝胶电泳法确定该抑制因子的分子量为10000dalton。变应性鼻炎时,因肥大细胞所释放的趋化因子的吸引,大量嗜酸性粒细胞游入鼻分泌物,该抑制因子可能参与其中蛋白酶的调节,它可能还有其他作用。这方面的
The authors used ~ 3H-casein as a substrate for the determination of protease and anti-protease activity in nasal secretions of patients with allergic rhinitis and chronic sinusitis. Found that chronic sinusitis purulent nasal secretions in the protease activity was significantly higher, while allergic rhinitis nasal secretions of this enzyme activity is particularly low. On the other hand, trypsin inhibitory activity in nasal secretions of allergic rhinitis was significantly higher than that of sinusitis (P <0.01). Note allergic rhinitis nasal secretions in the presence of a strong protease inhibitor. The molecular weight of this inhibitor was determined to be 10,000 dalton by SDS-polyacrylamide gel electrophoresis. In allergic rhinitis, a large number of eosinophils migrate into the nasal secretions due to the attraction of chemokines released by mast cells, which may be involved in the regulation of proteases therein and may have other effects. In this regard