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[目的]寻找快速、简单、成本低的高粱基因组DNA提取方法。[方法]首先,分别使用液氮研磨法、缓冲液研磨法、烘干研磨法和直接研磨法这4种方法从高粱叶片中提取基因组DNA,然后,通过凝胶电泳、SSR分析和SRAP分析检测所提DNA的浓度和纯度。[结果]采用4种方法提取高粱基因组DNA时的产量相差不大;采用前2种方法提取的DNA降解少,纯度高,可进行有效的SRAP-PCR和SSR-PCR,但缓冲液研磨法的SRAP-PCR结果稍差;而采用后两种方法提取的DNA降解严重,纯度低,但可进行有效的SSR-PCR。[结论]采用4种方法均可在短时间内提取到足够几十次PCR反应的高粱基因组DNA,液氮研磨法和缓冲液研磨法所提取的DNA应用范围更大,而烘干研磨法和直接研磨法所提取的DNA只能用于对片段较小的目的DNA进行特异性PCR。
[Objective] The research aimed to search for a rapid, simple and low-cost method for extracting genomic DNA from sorghum. [Method] Firstly, genomic DNA was extracted from leaves of Sorghum by liquid nitrogen milling method, buffer grinding method, drying and grinding method, and direct grinding method respectively. Then, the genomic DNA was extracted by gel electrophoresis, SSR analysis and SRAP analysis The DNA concentration and purity are given. [Result] The yield of sorghum genomic DNA extracted by four methods was similar. The DNA extracted by the first two methods had less degradation and high purity, and could be used for effective SRAP-PCR and SSR-PCR. However, The result of SRAP-PCR was a little worse. However, the DNA extracted by the latter two methods had serious degradation and low purity, but effective SSR-PCR. [Conclusion] The sorghum genomic DNA extracted by dozens of PCR reactions can be extracted in a short time by four kinds of methods. The DNA extracted by liquid nitrogen grinding method and buffer grinding method has more application scope. However, the dry grinding method and The DNA extracted by the direct milling method can only be used to perform specific PCR on fragments of smaller DNA.