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对三七花药愈伤组织的诱导进行了探索。在由不同浓度的2,4-D,BA和IAA组成的16种培养基上对三七花药进行了愈伤组织的诱导试验。结果显示:在MS基本培养基中附加2,4-D2 0mg/L+BA1 5mg/L+IAA0 5mg/L+蔗糖6%的条件下,花药愈伤组织的诱导率可达41 1%.本试验为三七花药培养体系的建立奠定了基础。
The induction of anther callus of Panax notoginseng was explored. The induction experiment of anther on the anther of Panax notoginseng was carried out on 16 kinds of media composed of 2,4-D, BA and IAA with different concentrations. The results showed that the induction rate of anther callus reached 41 1% under the condition of 2,4-D2 0 mg / L + BA1 5 mg / L + IAA0 5 mg / L + sucrose 6% It laid the foundation for the establishment of the anther culture system of Panax notoginseng.