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应用TaqDNA聚合酶(ThermusaquaticusDNApolymerase)直接对RNA进行反转录成cDNA第一链,然后用特异引物进行PCR扩增,结果表明,反转录达到AMV逆转录酶的效果,且可能得到比AMV逆转录酶更完整的cDNA第一链。
Reverse transcription of RNA into cDNA first strand using Taq DNA polymerase (Thermusaquaticus DNApolymerase) followed by PCR amplification with specific primers showed that reverse transcription reached the effect of AMV reverse transcriptase and that reverse transcription could be obtained as compared to AMV Enzyme more complete cDNA first strand.