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为了挖掘小麦近缘物种的高分子量麦谷蛋白新亚基和抗白粉病基因新类型,以小麦品种中国春和Alcedo(来自德国)为对照,对133份小麦-近缘物种染色体系进行了高分子量麦谷蛋白亚基(HMW-GS)分析和白粉病抗性调查。HMW-GS分析发现,25份材料与对照小麦的HMW-GS类型不同,其中,含外源染色体HMW-GS的有16份;亲本部分HMW-GS发生沉默的有2份;含外源染色体HMW-GS且亲本的部分HMW-GS发生沉默的有7份。在含外源HMW-GS的材料中,73.9%的HMW-GS来自于小麦近缘物种第1同源染色体,17.4%的HMW-GS来自第2、3和5同源染色体。白粉病抗性调查显示,尾状山羊草E#1、两芒山羊草2Mbi#1、沙融山羊草4Ssh#8、高大山羊草6Sl#3和簇毛麦5V#3S染色体上可能含有抗小麦白粉病新基因,值得进一步向小麦转育。本研究发现的新HMW-GS和潜在抗小麦白粉病新基因为利用这些资源进行小麦抗病育种与品质改良打下了坚实的基础。
In order to excavate the new HMW glutenin subunits and new powdery mildew resistance genes of wheat related species, 133 wheat-related species were stained with high molecular weight Analysis of glutenin subunit (HMW-GS) and powdery mildew resistance. The results of HMW-GS analysis showed that the HMW-GS types of 25 materials were different from that of the control wheat, including 16 exogenous chromosomes HMW-GS, 2 silencing HMW-GS parents, -GS and part of the parent HMW-GS silence occurred in 7 copies. Among the exogenous HMW-GS-containing materials, 73.9% of the HMW-GS was from the first homologous chromosome of wheat relatives and 17.4% of the HMW-GS was from the 2nd, 3rd and 5th homologous chromosomes. Powdery mildew resistance surveys showed that the resistance of powdery mildew in E # 1, the two sheep goat grass 2Mbi # 1, Sha Rong goat grass 4Ssh # 8, the tall goat grass 6Sl # 3 and the cluster hairy wheat 5V # 3S chromosome may contain anti-wheat The new gene of powdery mildew, it is worth to further transfer to wheat. The new HMW-GS and potential new wheat powdery mildew found in this study laid a solid foundation for the utilization of these resources in wheat disease-resistant breeding and quality improvement.