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的 观察麻醉诱导峰浓度的异丙酚、咪唑安定、依托咪酯及同芬太尼复合后,对心肌细胞钙离子移动的影响,研究其对心肌收缩力的作用。方法 用Fluo-3AM钙荧光指示剂染色急性分离的大鼠心肌细胞,在激光共聚焦显微镜下动态观察用药前和使用异丙酚(50μmol/L)、咪唑安定(3μmol/L)、依托咪酯(3μmol/L)及复合芬太尼(20ng/ml)后,KCl诱发的细胞内钙离子荧光强度的变化。结果 异丙酚明显抑制钙离子跨膜内流,细胞内钙荧光强度峰值较对照组下降15.3%(P<0.05),而咪唑安定、依托咪酯虽减慢钙的内流,但最终细胞内钙荧光强度与对照组无统计学差异(P>0.05)。复合芬太尼后,各组细胞内钙荧光强度升高的速度和峰值较单独使用该静脉麻醉药无统计学差异(P>0.05)。结论 复合使用芬太尼不会加重异丙酚、咪唑安定、依托咪酯对心肌细胞钙内流的抑制作用
To observe the effects of propofol, midazolam, etomidate and fentanyl on the myocardial calcium mobilization induced by anesthesia, and to study its effect on myocardial contractility. Methods Acutely isolated rat cardiomyocytes were stained with Fluo-3AM calcium fluorometric indicator. The cells were stained with 50μmol / L propofol (3μmol / L), etomidate (3μmol / L) and fentanyl (20ng / ml), KCl-induced intracellular calcium fluorescence intensity changes. Results Propofol obviously inhibited the transmembrane influx of calcium ions, and the peak value of intracellular calcium fluorescence was 15.3% lower than that of the control group (P <0.05). However, midazolam and etomidate decreased the influx of calcium, but the intracellular Calcium fluorescence intensity and the control group no significant difference (P> 0.05). After the combination of fentanyl, the intracellular calcium fluorescence intensity of each group increased faster than the peak value and the use of intravenous anesthetics alone was no significant difference (P> 0.05). Conclusion Combined use of fentanyl does not aggravate the inhibitory effect of propofol, midazolam and etomidate on intracellular calcium influx in cardiomyocytes