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利用人脐带血单个核细胞，联合粒／巨噬细胞集落刺激因子（GM－CSF）和肿瘤坏死因子-α（TNF－α），经2周左右可培养出大量树突状细胞（DCs）；来自正常人外周的单个核细胞，经GM－CSF和IL-4作用，8－10天后亦可培养出大量（4－5）×10~6／50ml）DCs。该研究为进一步研究DCs负载肿瘤抗原作为疫苗，为肿瘤免疫中的应用奠定了物质基础。
Using mononuclear cells of human umbilical cord blood, GM-CSF and tumor necrosis factor-α (TNF-α), a large number of dendritic cells (DCs) can be cultured after about 2 weeks; Mononuclear cells from normal human peripheral blood can be cultured in a large amount (4-5) × 10 ~ 6 / 50ml) after 8-10 days by GM-CSF and IL-4. This study lays the material foundation for the application of tumor immunity in order to further study the DCs loaded with tumor antigens as a vaccine.