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目的探讨基质蛋白酶-9(MMP-9)CD34在肝细胞癌的表达与侵袭转移过程中的表达关系。方法应用免疫组织化学SABC法,分析38例手术切除的肝癌标本MMP-9,CD34的变化,应用图象分析法进行定量分析MMP-9,应用血管记数分析CD34,并与临床病理相对照。结果 MMP-9的表达在有门脉癌栓或肝内转移者显著高于无门脉癌栓或肝内转移者。有门脉癌栓或肝内转移者11例,MMP-9在癌组织的表达量为8.07±0.48;无门脉癌栓或肝内转移者其表达量为5.04±0.22,P<0.05。在有侵袭转移情况下MMP-9在癌组织的表达量(8.07±0.48)显著高于癌旁组织(4.97±0.23),P<0.05 MMP-9在癌边缘组织的表达(8.56±0.60)显著高于癌旁组织(4.97±0.23),P<0.05,CD34的表达在有门脉癌栓或肝内转移者(96.65±17.65)显著高于无门脉癌栓或肝内转移者(74.25±15.25),P<0.05,在有侵袭转移情况下CD34在癌组织的表达量(96.65±17.65)显著高于癌旁组织(10.25±6.54),P<0.05。在无侵袭转移情况下CD34的表达在癌组织(74.25±15.25)、癌边缘组织(73.50±13.60)、癌旁组织(69.20±13.60)中无差异(P>0.05)。MMP-9CD34进行相关分析r=0.74,P<0.01为正相关。结论肝细胞癌在有侵袭转移情况下MMP-9在癌组织与癌边缘组织的表达明显增高。它与肿瘤的侵袭转移有关,它不仅破坏ECM而且调节血管生长使肿瘤容易扩散。而CD34在有侵袭转移情况下能反映肿瘤血管生长活跃,与肿瘤的侵袭转移有关。MMP-9的作用是促进肝细胞癌的侵袭转移,CD34可以反映肿瘤的血管情况。两者有正相关,MMP-9,CD34可作为判断肝细胞癌转移、预后的指标。
Objective To investigate the expression of matrix metalloproteinase-9 (MMP-9) CD34 in hepatocellular carcinoma and its relationship with invasion and metastasis. Methods Immunohistochemical SABC method was used to analyze the changes of MMP-9 and CD34 in 38 surgically resected hepatic carcinoma specimens. Image analysis was used to quantitatively analyze MMP-9. Vascular scores were used to analyze CD34 and compared with clinical pathology. Results The expression of MMP-9 in portal vein tumor thrombus or intrahepatic metastasis was significantly higher than those without portal vein tumor thrombus or intrahepatic metastasis. There were 11 cases with portal cancer thrombus or intrahepatic metastasis, and the expression level of MMP-9 in the cancer tissue was 8.07±0.48. The expression level of MMP-9 in the portal vein tumor thrombus or intrahepatic metastasis was 5.04±0.22, P<0.05. The expression of MMP-9 in cancer tissue was significantly higher than that in adjacent tissue (4.97±0.23) in invasion and metastasis (8.07±0.48), P<0.05 The expression of MMP-9 in cancer marginal tissue (8.56±0.60) was significant The expression of CD34 in portal vein tumor thrombus or intrahepatic metastasis (96.65±17.65) was significantly higher than that in non-cancerous tissue (4.97±0.23), P<0.05, compared with no portal vein tumor thrombus or intrahepatic metastasis (74.25± 15.25), P<0.05, the expression level of CD34 in cancer tissue (96.65±17.65) was significantly higher than that in the adjacent tissue (10.25±6.54) in invasion and metastasis, P<0.05. In the absence of invasion and metastasis, there was no difference in the expression of CD34 in cancer tissue (74.25±15.25), cancer marginal tissue (73.50±13.60), and adjacent tissue (69.20±13.60) (P>0.05). Correlation analysis of MMP-9CD34 was r=0.74, P<0.01 was a positive correlation. Conclusions The expression of MMP-9 in cancer tissue and cancer marginal tissue is significantly increased in hepatocellular carcinoma with invasion and metastasis. It is related to the invasion and metastasis of tumors. It not only destroys the ECM but also regulates the growth of blood vessels so that the tumors can easily spread. However, CD34 can reflect the active growth of tumor blood vessels in invasion and metastasis, which is related to the invasion and metastasis of tumors. The role of MMP-9 is to promote the invasion and metastasis of hepatocellular carcinoma, and CD34 can reflect the vascularity of the tumor. There is a positive correlation between the two, MMP-9, CD34 can be used as indicators to determine the metastasis and prognosis of hepatocellular carcinoma.