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目的：探索蛋白涂层支架携带质粒介导人肝脏诱导型一氧化氮合酶(iNOS)基因转染小型猪冠状动脉可行性。rn 方法：使用蛋白支架吸附去内毒素纯化质粒，以常规支架置入技术置入小型猪冠状动脉前降支中段。置入后第7天取出前降支置入段，分别提取总核糖核酸(RAN)并进行逆向多聚酶链反应(RT-PCR)，免疫组化染色检测导入人肝脏iNOS蛋白的表达。rn 结果：小型猪前降支置入支架处显示人iNOS基因信使核糖核酸(mRNA)转录，免疫组化染色显示中膜、内膜人iNOS基因表达人iNOS蛋白的颗粒，以平滑肌细胞最明显。rn 结论：蛋白涂层支架吸附去内毒素携带人iNOS基因质粒植入小型猪前降支冠状动脉，RT -PCR显示人iNOS基因的mRNA转录，免疫组化显示人iNOS蛋白的表达。“,”Objective:To assess the feasibility of plasmid-medicated local transfer of inducible nitric oxide synthase (iNOS) gene to coronary artery using protein-co ated metallic stents in mini-swine medel.rn Methods:The metallic stent was coated by cross-linked gelatin and mounted o n 3.0 mm Percutaneous transluminal coronary angioplasty (PTCA) balloon,then End otoxin-free ultrapure Endotoxin-free ultrapure Plasmid pcDNA3hepiNOS under the control of the comegalovirus (CMV) promoter/enhancer was absorbed on the stent. Protein-coated stainless steel stents were used as controls.All stents were imp lanted into the middle segment of left anterior descending artery through 7F lar ge luman gui ding catheter.(The ratio of balloon to vessel diameter was 1.1-1.3∶1).Total RNA of stented segment of coronary artery was extracted,its reverse transeriptio n polymerase chain reaction (RT-PCR) and immunohistochemical staining were perf ormed through routine methods.rn Results:At the 7th day after stenting,RT-PCR and immunohistochemical staining confirmed the expression of plasmid pcDNA3hepiNOS mRNA and presence of its protein at gene transered vessels (n＝2) but there was no expression in remote organs.Endothelialization of the vessel was observed in all animals throu gh scanning electromicroscopy.rn Conclusions:Local plasmid-mediated human inducible nitric oxide synthase ge ne transfer to coronary artery with protein-coated metallic stents is feasible in mini-swine model.