论文部分内容阅读
为研究垂体内分泌因子和局部因子(黄体多肽)的相互作用对牛早期黄体前列腺素F_(2α)(PGF_(2α))及孕酮(P)分泌的影响,分别选取牛处于早、中、晚及退化期的黄体4～7个,用于基因表达的研究。同时选取处于早期的黄体进行微透系统(MDS)的研究,每个实验选取5～8个卵巢黄体。用Trizol试剂通过Chomezynski和Sacchi方法分离牛黄体中的全部RNA,用RT-PCR分析测定黄体中的mRNA的含量。用MDS依次输入人类胰岛素样生长因子I(IGF-I)和催产素(OT)及生长激素(GH),牛黄体生成素(LH),每小时收集灌流液到10小时,收
In order to study the effect of the interaction between pituitary endocrine factor and local factor (luteal peptide) on the secretion of prostaglandin F_ (2α) (PGF_ (2α)) and progesterone (P) in early bovine, And degenerative luteal 4 to 7, for the study of gene expression. At the same time, we selected early corpus luteum to study the micro-penetrating system (MDS), and selected 5 to 8 ovary corpus luteum in each experiment. Total RNA in the bovine body was isolated by the Chomezynski and Sacchi methods using Trizol reagent and the content of mRNA in the luteal was determined by RT-PCR analysis. Human insulin-like growth factor I (IGF-I), oxytocin (OT), growth hormone (GH) and calf kidney endostatin (LH) were sequentially injected with MDS, and the perfusate was collected every hour for 10 hours