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目的研究纳洛酮(Nal)抗心律失常作用与其对压力反射敏感性(BRS)影响的关系.方法:以冠状动脉结扎法制备Sprague-Dewley大鼠急性心肌梗塞模型,心电图记录室性早搏(VE),室性心动过速(VT)和心室颤动(VF);静脉注射苯肾上腺素后同步记录的收缩压与心动周期变化的回归直线斜率(b)为BRS;TTC染色法评价梗死范围.结果:Nal 0.5 mg iv,小脑延髓池注射(ic)Nal 0.1mg和ic β-内啡肽(β-End)抗血清10 μL可抑制缺血性心律失常,心律失常积分为1.8±1.1(Nal iv)vs 3.8±2.1(生理盐水iv);1.7±1.5(Nal ic)vs 4.0±2.6(人工脑脊液ic)和1.7±1.6(β-End抗血清ic)vs4.1±2.0(血清ic)(P<0.05);并可加强BRS,BRS为4.2±1.8(Nal iv)vs 2.9±0.8(生理盐水iv);4.5±1.7(Nal ic)vs2.8±0.7(人工脑脊液ic)和4.4±1.1(β-End抗血清ic)vs3.0±0.9(血清ic)(P<0.05).BRS与心律失常积分呈负相关,r值分别为-0.69(Nal,iv);-0.72(Nal,ic)及-0.67(β-End抗血清.ic).P值均<0.05.结论:Nal通过拮抗中枢β-End及加强BRS起抗心律失常作用.
Objective To study the relationship between the antiarrhythmic effect of naloxone and its effect on the pressure sensitivity (BRS) .Methods: Acute myocardial infarction (AMI) model of Sprague-Dewley rats was prepared by coronary artery ligation, ), Ventricular tachycardia (VT), and ventricular fibrillation (VF). The linear regression slope (b) of systolic blood pressure and cardiac cycle changes recorded after intravenous injection of phenylephrine was BRS, and the infarct extent was evaluated by TTC staining. : Nal 0.5 mg iv, cerebellar cisterna injection (ic) Nal 0.1 mg and ic-endorphin 10 μL inhibited ischemic arrhythmia with an arrhythmia score of 1.8 ± 1.1 (Nal iv ) vs 3.8 ± 2.1 (saline iv); 1.7 ± 1.5 (Nal ic) vs 4.0 ± 2.6 (artificial cerebrospinal fluid ic) and 1.7 ± 1.6 (β-End antiserum ic) vs 4.1 ± 2.0 <0.05). BRS was also improved in patients with BRS of 4.2 ± 1.8 (Nal iv) vs 2.9 ± 0.8 (saline iv), 4.5 ± 1.7 (Nal ic) vs 2.8 ± 0.7 (artificial cerebrospinal fluid ic) and 4.4 ± 1.1 β-End antiserum ic) vs 3.0 ± 0.9 (serum ic) (P <0.05) .BRS was negatively correlated with the arrhythmia score and the r values were -0.69 (Nal, iv) And -0.67 (β-End antiserum .ic), all P <0.05.Conclusion: Pivoted β-End and strengthen BRS play antiarrhythmic effect.