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为高效利用桑黄资源开发多糖类抗氧化剂,以菌丝体生物量为考核指标,对桑黄菌丝体的液体摇瓶发酵培养条件进行单因素试验,筛选出较佳的培养条件:以葡萄糖为碳源,大豆蛋白胨为氮源,发酵液p H为6,培养温度为30℃,摇瓶转速为140 r/min,接种量(体积分数)为4%。采用超声波辅助复合酶法提取桑黄多糖,复合酶由3%纤维素酶(酶活力为1.0×10~4U/g)、2%木瓜蛋白酶(1.5×10~4U/g)和1.5%果胶酶(4.0×10~4U/g)组成,通过单因素试验优化提取工艺条件为料液质量浓度20 g/L、超声波功率300 W、处理温度50℃、处理时间45 min,在此条件下多糖得率可达10.867%。采用分光光度法测定6个来源菌株培养桑黄菌丝提取粗多糖的抗氧化活性,结果显示6种桑黄粗多糖样品均表现出较强的体外抗氧化活性,其中韩国来源菌株、中国金寨来源菌株的桑黄多糖对ABTS和DPPH自由基的清除能力最强,具有进一步开发为天然抗氧化剂和自由基清除剂的潜力。
In order to develop polysaccharides antioxidants efficiently using the Phellinus linteus resources, the mycelium biomass was taken as the assessment index to test the liquid culture conditions of Phellinus linteus mycelium by single factor experiment, and the better culture conditions were screened: Glucose as carbon source, soy peptone as nitrogen source, fermentation broth p H 6, culture temperature 30 ℃, shake flask speed 140 r / min, inoculum size (volume fraction) 4%. Phellinus igniarius polysaccharides were extracted by ultrasonic assisted enzyme digestion. The enzyme complex was composed of 3% cellulase (1.0 × 10-4 U / g), 2% papain (1.5 × 10-4 U / g) and 1.5% pectin (4.0 × 10-4 U / g). The optimized extraction conditions were as follows: the mass concentration of feed solution was 20 g / L, the ultrasonic power was 300 W, the treatment temperature was 50 ℃ and the treatment time was 45 min. Yield up to 10.867%. The anti-oxidative activity of crude polysaccharides extracted from Phellinus igniarius collected from six strains was determined by spectrophotometry. The results showed that the six samples of Phellinus igniarius showed strong antioxidant activity in vitro. Among them, the strains from Korea, Jinzhai, Phellinus igniarius from the source strain has the strongest ability to scavenge ABTS and DPPH free radicals and has potential for further development as a natural antioxidant and free radical scavenger.