transfectio相关论文
Objective To develop a safe and novel immunoadjuvant to enhance the immunity and resistance of animals against E. coli i......
AIM: To examine the effect of hepatitis C virus (HCV) structural mimics of regulatory regions of the genome on HCV repli......
Ebola virus infection causes severe hemorrhagic fever in human and non-human primates with high mortality. Viral entry/i......
Establishment and expression of recombinant human glial cell linederived neurotrophic factor and TNF
Objective:To investigate the interference and expression of human glial cell line-derived neurotrophic factor(hCDNF) and......
Effects of P-glycoprotein expression induced by ulinastatin on HK-2 cells damage induced by paraquat
Objective To investigate the protective effect of Pglycoprotein up-regulated by ulinastatin(UTI)on HK-2cells during para......
Objective:Id2 is a natural inhibitor of the basic helix-loop-helix(bHLH) transcription factors.Although it is well known......
Objective: Lung cancer has emerged as a leading cause of cancer death in the world. Current therapies are ineffective, t......
Objective Pancreatic cancer is one of the most deadly cancers,which is characterized by its high metastatic potential.S1......
目的 探讨LIM和SH3蛋白1(LASP-1)对人结直肠癌细胞株体内外增殖能力的影响.方法 用逆转录聚合酶链反应(RT-PCR)和Western blot方法......
目的研究RNA干扰沉默Fra-1基因表达对人膀胱癌T24细胞侵袭及迁移能力的影响。方法设计合成Fra-1特异性siRNA并转染人膀胱癌T24细胞......
目的 培养NIH/3T3细胞,并进行慢病毒载体介导的VEGF165基因转染,为建立转基因小鼠血管瘤模型奠定基础.方法 采取贴壁培养法分离培......
目的:构建血管生成抑制因子arresten基因的真核表达载体,并观察瘤内注射质粒DNA-脂质体复合物对裸鼠移植瘤生长的影响.方法:利用PC......
目的建立兔颞下颌骨关节病动物模型,观察携带有GFP-HGF cDNA基因体外转染兔髁突软骨细胞后对兔颞下颌骨关节病的治疗效果。方法以......
为探讨2型重组腺相关病毒（recombinant adeno-associated virus 2，rAAV-2）载体能否有效地转染神经于细胞（neural stemcell，NSC），本研究采......
基于新型树枝状大分子聚酰基硫脲(PATU),通过巯基-烯Michael加成反应得到表面N,N-二甲基半胱胺修饰的聚酰基硫脲(PATU-DMCA).核磁......
目的研究转染了逆转录病毒载体G1 CEACDNa致SW480肠癌细胞的死亡机制.方法脂质体法转染G1 CEACDNa至SW480细胞.行RT-PCR间接验证CD......
【目的】构建泛素启动酶Arkadia和泛素羧基末端水解酶-5（亦称UCH37）的表达载体，并探讨Smad7蛋白的泛素化修饰对马兜铃酸肾病中TGF-D1／S......
为进一步了解维拉帕米对抗药性逆转作用的特征，在人ＭＤＲ１基因传染的Ｓｗｉｓｓ－３Ｔ３多药抗药性细胞，观察了维拉帕米逆转幅度与阿霉素抗性水平的关系。讨论......
目的制备包封荧光素钠(FS)的脂质体,考察阳离子脂质材料(DC-chol)和聚乙二醇(PEG)对脂质体包封率、细胞转染率及膜流动性的影响.方......
目的 构建人肝细胞核因子-1α（hepatocyte nuclear factor1α，HNF1α）真核表达载体，在体外表达并检测。方法 提取HepG2总RNA，用RT-PCR方......
目的：探讨携带人肝细胞生长因子基因的重组腺病毒（Ad—HGF）修饰的骨髓间充质干细胞（MSCs）局部异体移植对烧伤创面愈合的作用。方法：密度......
目的：应用分子克隆技术，构建CYP2E1基因过表达载体，转染L02肝细胞，建立CYP2E1过表达细胞株并进行鉴定，为深入研究环境和职业毒物的毒作......
目的：探讨神经细胞粘附分子（neural cell adhesion molecule,NCAM)对人脑胶细胞株U251MG增殖行为的影响。方法：将外源性NACM－140质粒转......
目的观察转入野生型PTEN基因的胶质瘤细胞体外侵袭力改变,探索PTEN基因对胶质瘤细胞影响方式.方法构建野生型PTEN基因的pcDNA3.1 H......
[目的]优化脂质体介导外源基因体外转染牦牛乳腺上皮细胞的条件。[方法]通过胶原酶消化法和组织块贴壁法,体外分离培养得到牦牛乳......
本文介绍阳离子乳剂作为基因载体所具有的条件及目前关于其长循环和靶向性的研究。同时,与脂质体相比,阳离子乳剂在细胞转染方面的......
目的探讨心房颤动(AF)相关微小RNA-1266-5p(miR-1266-5p)与AF相关钠离子通道蛋白α亚基编码基因SCNA5的靶向调控关系。方法构建含......
前文已证实在体外（ｉｎｖｉｔｒｏ）实验接近生理环境的条件下，本室设计，合成并克隆到的核酶能够高铲选择性的定点切割Ｔ２４－ｒａｓ活化癌基因转录物，在此基础上，为阐......
In this paper, a novel series of bis [(aminoethyl)]-amine cationic lipid derivatives have been synthesized and identifie......
