transfectio相关论文
Objective To develop a safe and novel immunoadjuvant to enhance the immunity and resistance of animals against E. coli i......
AIM: To examine the effect of hepatitis C virus (HCV) structural mimics of regulatory regions of the genome on HCV repli......
Ebola virus infection causes severe hemorrhagic fever in human and non-human primates with high mortality. Viral entry/i......
Establishment and expression of recombinant human glial cell linederived neurotrophic factor and TNF
Objective:To investigate the interference and expression of human glial cell line-derived neurotrophic factor(hCDNF) and......
Effects of P-glycoprotein expression induced by ulinastatin on HK-2 cells damage induced by paraquat
Objective To investigate the protective effect of Pglycoprotein up-regulated by ulinastatin(UTI)on HK-2cells during para......
Objective:Id2 is a natural inhibitor of the basic helix-loop-helix(bHLH) transcription factors.Although it is well known......
Objective: Lung cancer has emerged as a leading cause of cancer death in the world. Current therapies are ineffective, t......
目的 探讨LIM和SH3蛋白1(LASP-1)对人结直肠癌细胞株体内外增殖能力的影响.方法 用逆转录聚合酶链反应(RT-PCR)和Western blot方法......
目的研究RNA干扰沉默Fra-1基因表达对人膀胱癌T24细胞侵袭及迁移能力的影响。方法设计合成Fra-1特异性siRNA并转染人膀胱癌T24细胞......
目的:构建血管生成抑制因子arresten基因的真核表达载体,并观察瘤内注射质粒DNA-脂质体复合物对裸鼠移植瘤生长的影响.方法:利用PC......
目的建立兔颞下颌骨关节病动物模型,观察携带有GFP-HGF cDNA基因体外转染兔髁突软骨细胞后对兔颞下颌骨关节病的治疗效果。方法以......
为探讨2型重组腺相关病毒（recombinant adeno-associated virus 2，rAAV-2）载体能否有效地转染神经于细胞（neural stemcell，NSC），本研究采......
基于新型树枝状大分子聚酰基硫脲(PATU),通过巯基-烯Michael加成反应得到表面N,N-二甲基半胱胺修饰的聚酰基硫脲(PATU-DMCA).核磁......
目的 构建人肝细胞核因子-1α（hepatocyte nuclear factor1α，HNF1α）真核表达载体，在体外表达并检测。方法 提取HepG2总RNA，用RT-PCR方......
目的：应用分子克隆技术，构建CYP2E1基因过表达载体，转染L02肝细胞，建立CYP2E1过表达细胞株并进行鉴定，为深入研究环境和职业毒物的毒作......
目的观察转入野生型PTEN基因的胶质瘤细胞体外侵袭力改变,探索PTEN基因对胶质瘤细胞影响方式.方法构建野生型PTEN基因的pcDNA3.1 H......
本文介绍阳离子乳剂作为基因载体所具有的条件及目前关于其长循环和靶向性的研究。同时,与脂质体相比,阳离子乳剂在细胞转染方面的......
