【摘 要】
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The cloning of large secondary metabolite biosynthetic gene clusters (20-210 kb) with high G+C content in actinomycetes is a significant challenge.In our work,
【机 构】
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KeyLaboratoryofSyntheticBiology,InstituteofPlantPhysiologyandEcology,ShanghaiInstitutesforBiological
论文部分内容阅读
The cloning of large secondary metabolite biosynthetic gene clusters (20-210 kb) with high G+C content in actinomycetes is a significant challenge.In our work, an efficient method modified from the Gibson assembly approach has been developed, which enables the assembly of large,high G+C DNA fragments with high efficiency.
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