Microfluidics has evolved from initially a scale-dependent chemical analysis technology towards a versatile platform that opens up new opportunities to addressing the challenges in biology and medicine.In particular,it provides a powerful tool to enable quantitative measurements of biomolecules and dynamic pathways across radically different spatiotemporal scales.In this talk,I will discuss the design and implementation of integrated microfluidic systems for quantitative molecular and cellular analysis down to the single cell/copy levels with high throughput.Their applications to high-performance detection of cancer-associated biomarkers,such as genetic mutations,proteins and circulating microvesicles,will be demonstrated.These results would suggest the feasibility of translating these microfluidics-enabled bioanalytical technologies into biomedical and clinical investigations.