【摘 要】
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Keywords: mesenchymal stem cells, osteogenic induction, alkaline phosphatase, biological security, tumorigenicity,chromosome karyotype Objective: Mesenchymal stem cells (MSCs), with versatile growth a
【机 构】
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Department of oral and maxillofacial surgery,Affiliated Stomatological Hospital of Guangxi Medical U
【出 处】
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11th Asian Congress on Oeal and Maxillofacial Surgery(第十一届亚洲
论文部分内容阅读
Keywords: mesenchymal stem cells, osteogenic induction, alkaline phosphatase, biological security, tumorigenicity,chromosome karyotype Objective: Mesenchymal stem cells (MSCs), with versatile growth and differentiation potential, are ideal candidates for tissue engineering and can be osteogenically-induced by ascorbic acid (AA), beta-glycerophosphate (βGP) and dexamethasone (DEX) ex vivo.Methold: In this study, different concentrations of AA, βGP and DEX were divided into 4 groups, and MSCs were induced into osteoblasts.Then, the Characteristic of osteoblasts was detected by gomori method for alkaline phosphatase (ALP).At the same time, the induced cells were injected into the subcutaneous tissue of nude mice for evaluation of tumorigenicity and chromosome G banding of the induced MSCs was used to analyze the karyotype which was used to observe whether there were chromosomal inversion, translocation, deletion, duplication or fusion.
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