论文部分内容阅读
Bombyx mori nucleopolyhedrovirus(BmNPV)ORF79(Bm79)encodes an occlusion-derived virus(ODV)-specific envelope protein,which is a homologue of the per os infectivity factor 4(PIF4)of Autographa californica multiple nucleopolyhedrovirus(AcMNPV).To investigate the role of ORF79 in the BmNPV life cycle,a Bm79 knockout virus(vBm Bm79KO)was constructed through homologous recombination in Escherichia coli.Viral DNA replication,budded virus(BV)production and polyhedra formation were unaffected by the absence of BM79.