【摘 要】
：
Objective To evaluate the in vitro biological effect of oseltamivir carboxylate against varied subtype influenza viruses isolated from east china during 2004-2006 influenza season, and then found out
【机 构】
：
Department of Epidemiology, School of Public Health, Fudan University, Room 8-417 130 Dong An Rd., S
论文部分内容阅读
Objective To evaluate the in vitro biological effect of oseltamivir carboxylate against varied subtype influenza viruses isolated from east china during 2004-2006 influenza season, and then found out the sensitivity and drug-resistant variant.Methods Influenza viruses were isolated from flu-like cases during influenza season in Shanghai, Wuxi and Deqing.50 isolations including 3 subtypes were randomly selected in this study.The anti-viral activity of oseltamivir carboxylate was detected by employing neutral-red uptake assay.The 50% inhibition concentration (IC50) served as the index of anti-viral effect.
其他文献
Objective:To evaluate the clinical outcome and prognosis of expansive open-door laminoplasty for cervical spondylosis.Methods:36 patients who underwent expansive open-door laminoplasty for cervical sp
Objective:To treat the cervical diseases caused by any pathogics though posterior approach for cervical fixation.Method:18 patients have underwent posterior fixation of cervical spine since three year
Objective:To development a three-dimensional finite element model of normal total adult human pelvis and to establish a platform for biomechanical research of human pelvis.Methods:A set of CT images o
Objectives:To determine the initial stability of a novel construct in one-level cadaveric cervical spine model by comparing it with a conventional method.Methods:Twelve fresh human cadaveric cervical
Objective:To investigate the osteogenic potential of gene activated nanobone putty.Methods:Twenty-four Kunming mice were used in this experiment.The mice were divided into two groups randomly.The nano
自2003年我至今，我们在广州市儿童医院进行了较大量的儿童呼吸道和肠道疑似病毒感染临床样品的实验室检测分析，主要采用的技术方法是病毒培养、荧光PCR和ELISA抗原检测技术，结果提示：荧光PCR或RT-PCR、病毒抗原ELISA检测可以作为部分上呼吸道病毒感染的病原学临床检测应用；胶体金快检模式的病毒抗原检测可以用于肠道病毒感染的病原学检测；临床送检样品的采取、运输和保存方法在临床检测中很重要、需
Human astrovirus (HAstV) is the second most common causes for viral gastroenteritis in young children.This study was undertaken to investigate the molecular epidemiology and the genomic characterizati
目的探讨悬浮阵列技术在儿童下呼吸道病毒感染诊断中的应用.方法采用Tem-PCR技术对80例急性下呼吸道感染患儿的鼻咽分泌物标本,同时扩增呼吸道合胞病毒(RSV)、副流感病毒(PIV)、流感病毒A (INFA)、流感病毒B(INFB)、SARA冠状病毒(SARS Cov)鼻病毒(RhV)、柯萨奇及埃可病毒(CVEV)、人类偏肺病毒(hMPV)的特异性RNA.扩增产物经多功能悬浮点阵仪检测.结果80例
目的 了解云南无偿献血员HIV-1感染者的亚型多态性和原发耐药流行状况.方法分析了云南昆明血液中心2005～2006年HIV阳性血浆53份,提取RNA,巢式PCR扩增Pol(含蛋白酶区1～99氨基酸全长和逆转录酶区1～300氨基酸)基因区,测序分析后,使用Los Alamos标准株,构建系统进化树分析亚型；使用斯坦福大学HIV Drug Resistance Database分析耐药相关突变.结果