【摘 要】
:
目的探讨悬浮阵列技术在儿童下呼吸道病毒感染诊断中的应用.方法采用Tem-PCR技术对80例急性下呼吸道感染患儿的鼻咽分泌物标本,同时扩增呼吸道合胞病毒(RSV)、副流感病毒(PIV)、流感病毒A (INFA)、流感病毒B(INFB)、SARA冠状病毒(SARS Cov)鼻病毒(RhV)、柯萨奇及埃可病毒(CVEV)、人类偏肺病毒(hMPV)的特异性RNA.扩增产物经多功能悬浮点阵仪检测.结果80例
【机 构】
:
深圳儿童医院呼吸科 深圳市捷纳生物技术有限公司 518026
论文部分内容阅读
目的探讨悬浮阵列技术在儿童下呼吸道病毒感染诊断中的应用.方法采用Tem-PCR技术对80例急性下呼吸道感染患儿的鼻咽分泌物标本,同时扩增呼吸道合胞病毒(RSV)、副流感病毒(PIV)、流感病毒A (INFA)、流感病毒B(INFB)、SARA冠状病毒(SARS Cov)鼻病毒(RhV)、柯萨奇及埃可病毒(CVEV)、人类偏肺病毒(hMPV)的特异性RNA.扩增产物经多功能悬浮点阵仪检测.结果80例标本中,检出阳性23例,检出率为28.8%(23/80).21例为单一病毒感染,其中RSV 8例,PIV 4例,INFA 4例,RhV 2例, CVEV 1例,hMPV2例;2例为混合感染,1例为PIV和CVEV双重感染,另1例为PIV、CVEV和RhV三重感染.结论Tem-PCR多重扩增技术结合多功能悬浮阵列检测平台,能够同时快速检测临床标本中呼吸道多种RNA病毒,适用于呼吸道病毒感染的分子诊断及鉴别诊断.
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