【摘 要】
：
目的探讨悬浮阵列技术在儿童下呼吸道病毒感染诊断中的应用.方法采用Tem-PCR技术对80例急性下呼吸道感染患儿的鼻咽分泌物标本,同时扩增呼吸道合胞病毒(RSV)、副流感病毒(PIV)、流感病毒A (INFA)、流感病毒B(INFB)、SARA冠状病毒(SARS Cov)鼻病毒(RhV)、柯萨奇及埃可病毒(CVEV)、人类偏肺病毒(hMPV)的特异性RNA.扩增产物经多功能悬浮点阵仪检测.结果80例
【机 构】
：
深圳儿童医院呼吸科 深圳市捷纳生物技术有限公司 518026
论文部分内容阅读
目的探讨悬浮阵列技术在儿童下呼吸道病毒感染诊断中的应用.方法采用Tem-PCR技术对80例急性下呼吸道感染患儿的鼻咽分泌物标本,同时扩增呼吸道合胞病毒(RSV)、副流感病毒(PIV)、流感病毒A (INFA)、流感病毒B(INFB)、SARA冠状病毒(SARS Cov)鼻病毒(RhV)、柯萨奇及埃可病毒(CVEV)、人类偏肺病毒(hMPV)的特异性RNA.扩增产物经多功能悬浮点阵仪检测.结果80例标本中,检出阳性23例,检出率为28.8％(23/80).21例为单一病毒感染,其中RSV 8例,PIV 4例,INFA 4例,RhV 2例, CVEV 1例,hMPV2例；2例为混合感染,1例为PIV和CVEV双重感染,另1例为PIV、CVEV和RhV三重感染.结论Tem-PCR多重扩增技术结合多功能悬浮阵列检测平台,能够同时快速检测临床标本中呼吸道多种RNA病毒,适用于呼吸道病毒感染的分子诊断及鉴别诊断.
其他文献
Objective:To compare the clinic outcome between cervical spine stability reconstruction and vertebral artery adventitia decollement decompression in treating vertebral artery type of cervical spondylo
Objective:To discuss the value of anterior segmental decompression,bone grafting and internal fixation in the treatment of multi-segmental cervical spondylotic myelopathy.Method:In group A,there were
Objective:To analyze the causesof complications following expansive single open-door laminoplasty and to find out about the way for prevention and treatment of those complications.Methods:Research com
Objective:To evaluate the clinical outcome and prognosis of expansive open-door laminoplasty for cervical spondylosis.Methods:36 patients who underwent expansive open-door laminoplasty for cervical sp
Objective:To treat the cervical diseases caused by any pathogics though posterior approach for cervical fixation.Method:18 patients have underwent posterior fixation of cervical spine since three year
Objective:To development a three-dimensional finite element model of normal total adult human pelvis and to establish a platform for biomechanical research of human pelvis.Methods:A set of CT images o
Objectives:To determine the initial stability of a novel construct in one-level cadaveric cervical spine model by comparing it with a conventional method.Methods:Twelve fresh human cadaveric cervical
Objective:To investigate the osteogenic potential of gene activated nanobone putty.Methods:Twenty-four Kunming mice were used in this experiment.The mice were divided into two groups randomly.The nano
自2003年我至今，我们在广州市儿童医院进行了较大量的儿童呼吸道和肠道疑似病毒感染临床样品的实验室检测分析，主要采用的技术方法是病毒培养、荧光PCR和ELISA抗原检测技术，结果提示：荧光PCR或RT-PCR、病毒抗原ELISA检测可以作为部分上呼吸道病毒感染的病原学临床检测应用；胶体金快检模式的病毒抗原检测可以用于肠道病毒感染的病原学检测；临床送检样品的采取、运输和保存方法在临床检测中很重要、需
Human astrovirus (HAstV) is the second most common causes for viral gastroenteritis in young children.This study was undertaken to investigate the molecular epidemiology and the genomic characterizati