【摘 要】
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Objective : To investigate the impact of Jianpi Huayu recipes on Smad7 protein and epithelial mesenchymal transition(EMT)essential protein(E-cadherin/N-cadherin)of hepatocellular carcinoma cell.Method
【机 构】
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Department of Hepatobilliary Surgery,the First Affiliated Hospital of Guangzhou University of Chines
【出 处】
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第五届广州国际肿瘤学会议暨第一届中国抗癌协会肿瘤靶向治疗专业委员会年会
论文部分内容阅读
Objective : To investigate the impact of Jianpi Huayu recipes on Smad7 protein and epithelial mesenchymal transition(EMT)essential protein(E-cadherin/N-cadherin)of hepatocellular carcinoma cell.Methods:Hepatocellular carcinoma cell line MHCC97-H was cultured in different concentrations(10%and 20%)of “Jianpi Huayu Therapy” medicated serum and 10%blank serum,respectively.Real-time PCR(qPCR)and western blot was used to detect the mRNA level of Smad7 and Snail,and protein level of Smad7,Snail,E-cadherin,and N-cadherin,respectively.Wound healing assays and Transwell chamber was respectively used to detect the invasion and migration ability of MHCC97-H cell.
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