【摘 要】
：
Background Inactivating MEN1 mutations are the most common genetic defects present in sporadic and inherited pancreatic neuroendocrine tumors (PNETs).The lack of interventional therapies prompted us t
【机 构】
：
Shanghai Clinical Center for Endocrine and Metabolic Diseases, Rui-Jin Hospital, Shanghai Jiao-Tong
【出 处】
：
中华医学会第十四次全国内分泌学学术会议
论文部分内容阅读
Background Inactivating MEN1 mutations are the most common genetic defects present in sporadic and inherited pancreatic neuroendocrine tumors (PNETs).The lack of interventional therapies prompted us to explore the therapeutic approach of targeting β-catenin signaling in MEN1 mutant PNETs.Methods The phosphorylation and activation of β-catenin signaling were analyzed in human and mouse menin-deficient PNETs.Pancreatic β-cell-specific menin and β-catenin knockout and double knockout mice were used to assess the tumorigenesis, survival rate and metabolic phenotypes.Pancreas perfusion and islet transplantation in mouse models were conducted to validate the consequence of β-catenin blockage.
其他文献
Background Many epidemiological studies indicated that prolonged high iodine intake would lead to an elevation of serum thyrotrophin (TSH) level, but the mechanism is elusive.Type 2 iodothyronine deio
Objective To investigate the sensitivity and specificity of the different Growth hormone stimulation tests(GHSTs) in children with short stature, and the influence factors on peak growth hormone(GH) r
Aims/hypothesis Estradiol (E2) and progesterone (P＜/span＞4) have been reported to modulate glucose metabolism during pregnancy.In this study, we aimed to evaluate the role of TCF7L2, a key regulator o
Objective Glucocorticoids are administered in moderate and severe cases of subacute thyroiditis (SAT),providing markedly relief from fever and pain.To our knowlage, there have been no reports regardin
目的 明确miR-17～92在胰岛β细胞功能调控中的作用以及机理方法 1)通过Cre-loxp系统构建胰岛β细胞特异性敲除miR-17～92基因的基因工程鼠;常规监测小鼠代谢相关指标;测量基因敲除鼠在体和离体的胰岛素分泌情况;观察胰腺的组织形态学并定量计算β细胞数量、体积、质量,胰岛β细胞的凋亡、增殖能力;荧光定量RT-PCR检测胰岛细胞胰岛素合成分泌相关基因表达情况.Western实验检测PTE
目的:评估利格列汀在2型糖尿病合并冠状动脉疾病患者中的安全性和疗效方法:2型糖尿病(T2D)患者的冠心病(CAD)的发病率和死亡率升高.我们纳入一些随机、安慰剂(PBO)对照研究数据,对DPP-4抑制剂利格列汀(LINA)5 mg治疗CAD患者的患者安全性和疗效数据(采用SMQ查询"栓塞和血栓事件"定义)进行了考察.安全性分析集包括治疗持续时间≥12周的患者(19项试验;LINA,n=451;PB
目的 全基因组关联分析发现KLF14基因与2型糖尿病密切相关.然而KLF14与糖代谢和胰岛素信号通路的关系尚不明确,本文旨在阐明其相互作用关系.方法 基因mRNA和蛋白表达分别用Q-PCR和western blot检测;2-脱氧葡萄糖法测定糖摄取;利用western blot法测定胰岛素信号通路水平变化.
目的 了解重庆某社区人群十年来糖尿病及糖尿病前期患病率的变化趋势.方法 调查分别于2003年、2008年及2013年在重庆市沙坪坝区某社区进行,包括人体学测量、空腹血糖检验和口服75g无水葡萄糖耐量试验(oral glucose tolerance test,OGTT).糖尿病及糖尿病前期根据世界卫生组织(World Health Organization,WHO)1 999年诊断标准诊断.被调查
目的 在前期研究基础上,探讨可导致迟发DM的突变前胰岛素原R6C和R6H的无效转位导致β细胞衰竭和DM的分子机制方法 1在有无MG132处理下,用35S-Met/Cys标记追踪转染R6C/A24D的293T,INSAb-IP,SDS-PAGE分析2设计恢复n/c区正电荷的双突变R6C-M5R和R6C-D20R转染293T细胞,用35SCys/Met标记,INSAb-IP,SDS-PAGE分析3应用
Background and aims Dipeptidyl peptidase IV (DPP-IV),whose substrates includes glucagon-like peptide 1 and many other polypeptides, is not only important in pancreatic β-cell regulation but also has c