Immortalization of oral mucosal epithelial cells though hTERT with Bmi-1 up regulation and p16 down

来源 :11th Asian Congress on Oeal and Maxillofacial Surgery(第十一届亚洲 | 被引量 : 0次 | 上传用户:kxianwen
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Objective: To establish the immortalized oral mucosal epithelial (OME) cell line by transfection with human telomerase reverse transcriptase (hTERT).Methods: Oral mucosal epithelial cells were infected with retrovial vector encoding hTERT.Infected cells were selected with G418 and checked by immunocytochemistry (ICC), in vitro proliferation, the ability of tumorigenesis, RT-PCR and Western blot detect the expression ofhTERT.
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