Qinghai Lake, a typical depositional salt lake, is on the Northeast of Qinghai-Tibetan Plateau.Qinghai Lake is the most biggest inland saltwater lake in China, and its special environment, such as low temperature, hypoxia, high altitude, strong solar radiation, large difference in temperature, produces some particular biological resource.In this study, halophile fungi of Qinghai Lake were investigated by using traditional culture-dependent methods.Totally, 617 fungal cultures were isolated from different regions of QingHai Lake (Heima Lake, Erhai and Gahai).Most of them were assigned to 18 genera and 45 species based on morphological and molecular characterization.The number of strains of isolate of Ascomycetes was larger than that of Basidiomycetes and Zygomycetes in Qinghai lake.The most abundant genera were Cladosporium and Penicillium.Among them, 19 species and 25 species were assigned to slight halophile and moderate halophile, respectively, only one species was extreme halophile.Ma, H, and J of halophile fungi isolated from Heima Lake is larger than that of Erhai and Gahai.The possible reasons is that Heima Lake is the main resource of water in Qinghai Lake.Because of that 1.5% salinity is close to the true salinity of Qinghai Lake, Ma, H, and J of halophile fungi isolated from 1.5% salinity is larger than that of 10% and 30%.Ma and J of halophile fungi isolated from water were larger than soil and mud, while H, is lower than mud.It maybe concerned that the flow ability of water caused the result.This is a systematic report of cultural fungi from Qinghai Lake and halophile fungal diversity exists in Qinghai Lake.