【摘 要】
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Giardia duodenalis is a common and widespread intestinal protozoan parasite of both humans and animals.Previous epidemiological and molecular studies have identified Giardia infections in different an
【机 构】
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College of Animal Science and Veterinary Medicine,Henan Agricultural University,Zhengzhou 450002,Hen
【出 处】
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中国畜牧兽医学会兽医寄生虫学分会第十三次学术研讨会
论文部分内容阅读
Giardia duodenalis is a common and widespread intestinal protozoan parasite of both humans and animals.Previous epidemiological and molecular studies have identified Giardia infections in different animals and humans,but only limited information is available about the occurrence and genotypes of Giardia in cattle in China.In this study,we determined the occurrence of giardiasis and genetically characterized G.duodenalis in dairy cattle in Henan Province,central China.The overall prevalence of G.duodenalis was 7.2%(128/1777)on microscopic analysis,with the highest infection rate(22.7%)in calves aged less than 1 month.G.duodenalis assemblages and subtypes were identified with multilocus genotyping based on the SSU rRNA,β-giardin(bg),glutamate dehydrogenase(gdh),and triosephosphate isomerase(tpi)genes.Two assemblages were detected in the successfully sequenced samples: assemblage A(n =58),assemblage E(n =21),with a mixed E and A assemblage(n =2).Four novel subtypes of the gdh gene and seven of the bg gene were found among the G.duodenalis assemblage E isolates.Using the nomenclature for the multilocus genotype(MLG)model,nine novel multilocus genotypes E(MLGs E1-E9)and three MLGs A(a novel subtype AⅠ,previously detected subtype AⅡ-1,and a combination of both)were identified.MLG AⅡ-1 identified in this study may be an important zoonotic subtype.The dairy cattle in Henan are a potential public health concern.
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