Multidrug resistance is one major obstacle for successful chemotherapy in gastric cancer,yet the underlying mechanisms are still not clarified.Glycosylation,one of the most important post translational modifications of proteins,plays a vital role in diverse aspects of tumor progression.In the present study,we applied two multidrug resistance cell lines and their parental drug sensitive gastric cancer cell line to a modified glycoproteomics analysis strategy to investigate the MDR-related glycoproteomics changes in gastric cancer.