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Dermal irritation is the production of reversible damage to the skin following application of a test substance for up to 4 hours (OECD, 2002).Skin irritation is assessed by applying the test substances in a single dose to the skin.Prevalidation and validation studies have been reported that in vitro tests employing reconstructed human epidermis models are able to reliably discriminate between irritants and non-irritants according to EU classification system.Following the ECVAM Skin Irritation Validation Study (SIVS), the Episkin test method provided sufficient intra and inter-laboratory reproducibility and showed sufficient sensitivity and specificity for a standalone method to fully replace the in vivo skin irritation test in rabbits.The EpiSkinTM model, commercially available at www.skinethic.com, is a fully differentiated three-dimensional skin model comprising a reconstructed epidermis with a functional stratum corneum.